For example: citric acid. The efficiency of the product process and the dificulty and cost of the recoveryand purification processes are tigthly coupled. Fermentation 2. Cell removal and concentration 3. Cell disruptiuon 4. Removal of cell debris 5. Protein precipitation or aqueoust wo-phase extraction 6.
Ultrafiltration 7. Cromatographic purification 8. Solvent precipitation 9. Dialysis Lyophilization In the step above, can be generalized to involve four primary function: 1. Separation of insoluble product and other solids.
Primary isolation or concentration of product and removal of most of the water. Purification or removal contaminating chemical. Product preparation such as drying. Separation of Insoluble Products The separation of solid such as biomass insoluble particles and macromolecules from the fermentation broth is usually the first step in product recovery. For examples of pretreatment are heat treatment, pH and ionic strength adjustment, and the adition of coagulans and flocculants.
The major methods 1. Filtration both rotary vacuum filtration and micro- or ultrafiltration 2. Sentrifugation 3. Coagulation and flocculation. Filtration Is probably the most cost-efective method for the separationoflarge solid particles and cells from fermentation broth.
Continous rotary filters or rotary vacuum precoat filters are the most widly used types in the fermentation industry Filtration is commonly used for separating my celium from fermentation broth in anti biotic fermentations. It is also commonly used in waste-water treatment facilities. Centrifugation Is used to separate particles of size between and 0,1 m from liquid by centrifugal process. Open navigation menu. Close suggestions Search Search. User Settings. Skip carousel.
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Uploaded by Ganesh V Gaonkar. Document Information click to expand document information Description: Product recovery and purification, such as centrifugal, chromatography, crystallization, dialysis, drying, electroscopes, filtration, precipitation, etc. Did you find this document useful? Is this content inappropriate? Report this Document. Description: Product recovery and purification, such as centrifugal, chromatography, crystallization, dialysis, drying, electroscopes, filtration, precipitation, etc.
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